Effect of Fluoxetine Hydrochloride on Routine Metabolism of Lambari (Deuterodon iguape, Eigenmann, 1907) and Phantom Shrimp (Palaemon pandaliformis, Stimpson, 1871)

HIGHLIGHTS Fluoxetine increases the metabolic rate and excretion of ammonia in both species. O:N ratio in fish showed higher values in the highest concentrations of fluoxetine. The LC50 - 96 hour values of Palaemon pandaliformis represented greater toxicity. Both species are a good biological model for fluoxetine exposure studies.

Abstract Fluoxetine is an emerging pollutant that acts as a selective serotonin reuptake inhibitor (SSRI) and being a hydrolytic molecule that is photolytically stable and accumulaties in biological tissues, its disposal in the aquatic environment can interfere with the physiology of fish and shrimp. Thus, the objective of this study was to analyze the effects of fluoxetine on routine metabolism (metabolic rate, specific ammonia excretion and O:N ratio) of Deuterodon iguape and Palaemon pandaliformis. For this, five groups of each species, were exposed to different concentrations of fluoxetine for 24 hours (D. iguape) and 2 hours (P. pandaliformis). The results demonstrated that in D. iguape exposure to fluoxetine significantly increased both the metabolic rate by 75%, 85%, 55% and 50% for concentrations of 0.05; 0.1; 0.5 and 1.0 mgL-1, respectively, and the specific ammonia excretion by 40%, 48% and 20% for concentrations of 0.05; 0.1 and 0.5 mgL-1, respectively, when compared with their control. The O:N ratio was statistically greater in concentrations of 0.5 and 1.0 mgL-1. Concerning P. pandaliformis, exposure to fluoxetine increased metabolic rate at concentrations 30.0 and 60.0 µgL-1, and also increased specific ammonia excretion at concentrations 10.0, 30.0 and 60.0 µgL-1, when compared with the control group. It was concluded that exposure to fluoxetine increases the routine metabolism of both species and that at the concentration 1.0 mgL-1, Deuterodon iguape required different energy substrates.

Microbiology as an indicator of environmental health of Ilha Comprida lagoons- SP / Microbiologia como indicador da saúde ambiental das lagoas de Ilha Comprida - SP

With an area of 150 Km², the municipality of Ilha Comprida has limited sources of freshwater, sediments and nutrients. However, the sanitary aspects, aiming at Public Health, should be evaluated by determining the densities and the distribution of fecal pollution indicators, mainly bacteria from the group of thermotolerant coliforms. Thus, the objective of this work was to analyze the seasonal variations in the densities of thermotolerant coliforms in the lagoons of the municipality of Ilha Comprida (SP). For that, water samples were collected, in the period between 2014 and 2016, in lagoons of 13 localities (Juruvaúva, City Mar Sul, City Mar Norte, São Januário, Jardim Portugal, Atlântico, Icaraí, Adriana Sul, Adriana Norte, Porto Velho 2, Xandú, Jardim da Barra, Laguna Ponta da Praia) located in the municipality of Ilha Comprida (SP). The results showed that 21.90% of the samples were above the limit established by CONAMA. High densities of bacteria were found in the Icaraí lagoon. The highest averages of thermotolerant coliform densities were recorded in summer and spring. It is concluded that the seasonal variation interferes with the density of thermotolerant coliforms

Com uma área de 150 km², o município de Ilha Comprida tem fontes limitadas de água doce, sedimentos e nutrientes. No entanto, os aspectos sanitários, que visam a Saúde Pública, devem ser avaliados determinando as densidades e a distribuição de indicadores de poluição fecal, principalmente bactérias do grupo de coliformes termotolerantes. Assim, o objetivo deste trabalho foi analisar as variações sazonais nas densidades de coliformes termotolerantes nas lagoas do município de Ilha Comprida (SP). Para isso, foram coletadas amostras de água, no período entre 2014 e 2016, em lagoas de 13 localidades (Juruvaúva, Cidade do Mar do Sul, Cidade do Mar do Norte, São Januário, Jardim Portugal, Atlântico, Icaraí, Adriana Sul, Adriana Norte, Porto Velho 2, Xandú, Jardim da Barra, Laguna Ponta da Praia), localizada no município de Ilha Comprida (SP). Os resultados mostraram que 21,90% das amostras estavam acima do limite estabelecido pelo CONAMA. Foram encontradas altas densidades de bactérias na lagoa de Icaraí. As médias mais elevadas das densidades de coliformes termotolerantes foram registradas no verão e na primavera. Conclui-se que a variação sazonal interfere com a densidade de coliformes termotolerantes